The structured organization provides fixed spacing of sequencing clusters, making the flow cells less susceptible to overloading, and reduces run time (no need to map cluster sites during sequencing). Flow cell scratches (right). As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Please send the tracking information to next-gen@umn.edu. The DRAGEN Germline Pipeline v3 aligns and optionally variant calls FASTQ files, outputting a BAM, VCF, or both. Bio-IT Platform, TruSight The NovaSeq produces record output per flow cell combined with reduced on-instrument sequencing durations, thus dramatically improving throughput and speed over previous generations of sequencers. Discuss best practices, troubleshoot, and collaborate with Illumina scientists, developers, and each other. 0.05% Tween 20 wash solution: To a 500 ml centrifuge bottle . Not for use in diagnostic procedures (except as specifically noted). Yes, please consult with UMGC staff before submitting client-made libraries by emailing next-gen@umn.edu. . Sequence Hub, BaseSpace Building on the proven HiSeq 2500 System and leveraging innovative patterned flow cell technology, the HiSeq 4000 system provides improved speed and performance. Run times are based on running 2 flow cells of the same type; starting two different flow cells will impact run time. Please see our Bioanalyzer & Tapestation pricing for more information. MSI does not charge for SURFS storage costs, but files expire and are removed one year after they've been delivered. Impactful discoveries are within reach. Figure 1. Both the systems can work with either one or two flow cells. refer to Staggered Start of Runs in the NovaSeq 6000 Sequencing System Guide. The new kit includes a new version of SBS and PE reagent cartridges along with the proven NovaSeq flow cells and buffer cartridges. The NovaSeq 6000 system performs whole-genome sequencing efficiently and cost-effectively. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Detect coding and multiple forms of noncoding RNA in normal or low-quality samples. At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. NovaSeq performs whole-genome sequencing more efficiently and cost-effectively than ever. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This robust, scalable system has been adopted by leading research centers and featured in countless publications. The NovaSeq 6000 system performs whole-genome sequencing efficiently and cost-effectively. A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing or tumor-normal sequencing. Host: https://www.illumina.com | Remove any residual alcohol by wiping with a clean, dry lint-free tissue. *Time per sample, assuming 30x coverage/sample. Interpret results, get insights, and drive discovery with BaseSpace Variant Interpreter, and TruSight Software Suite. Its tunable output generatesup to 6 Tb and20B single reads in dual flow cell mode with streamlined workflows. See whats possible with the proven NovaSeq 6000 system and NGS technology, Rady Childrens Institute for Genomic Medicine is using the DNA PCR-Free Prep kit for NovaSeq 6000 to make a potentially record-breaking time-to-diagnosis for children with rare genetic disorders, Cole Trapnell, PhD is transforming the world of biology by making bioinformatics tools available to all, OmniSeq, Inc. scaled up to process a large number of both tissue and blood plasma samples every week, batching from 16 to 192 samples in a single run on the NovaSeq 6000, Genomics Englands human genome initiative (GenOMICC) has launched a study aimed at uncovering genetic factors in susceptibility to COVID-19, The DRAGEN platform enables GeneDx to scale to whole-genome analysis and identify variants with precision. 2022 Illumina, Inc. All rights reserved. For further information, search for Knowledge Base article What should I do if the NovaSeq 6000 flow cell cannot be loaded during run setup? 612-625-7736. The NovaSeq 6000 is the latest production-scale sequencer from Illumina generating unprecedented output in less than two days. Required Reagents and Consumables for Maintenance Wash: 1. Software Restriction Policy (SRP) on the NovaSeq 6000. NovaSeq 6000 storage management and best practices. For a dual flow cell 2x101 cycle run (200 Gb) on the HiSeq 2000 using HCS v1.3 and prior, you can expect 2 TB of intensity data (optionally transferred . Retailer Reg: 2019--2018 | This robust, scalable system has been adopted by leading research centers and featured in countless publications. Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity. NovaSeq 6000 flow cell types and their compatibility with NovaSeq Control Software versions. A NovaSeq 6000 running at its highest output will generate six terabytes (Tb) of data in 44 hours. NovaSeq 6000 flow cell types and their compatibility with NovaSeq Control Software versions. External clients may have data shipped on a hard drive purchased by the UMGC and invoiced to the client at a cost of $250 per hard drive. After the screen goes blank, press the toggle power switch on the back of the instrument to the OFF position (Figure 2). NovaSeq 6000 v1.5 reagent kits are now available. Analysis, Biological Data Apps, DRAGEN With the NovaSeqXP workflow, each flow cell lane can be loaded individually to separate different projects or methods between lanes. Address of host server location: 5200 Illumina Way, San Diego, CA 92122 U.S.A. All trademarks are the property of Illumina, Inc. or their respective owners. Apps, DRAGEN Cosmetic defects such as these rarely affect output or quality, and performance is guaranteed. The server can store as many runs as disk space permits. Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility. Troubleshooting Focus Camera (FTM) system check . *2.5 days total prep time, 6 hours hands-on time. Join a community of like-minded customers using Illumina sequencers, library preparation kits, and automated data analysis to fuel groundbreaking research. Patterned Flow Cell Technology. With the NovaSeq 6000 System, you can sequence up to 400 transcriptomes in a single run using a dual S4 flow cell. Before loading the flow cell on the NovaSeq 6000, inspect the flow cell and NovaSeq 6000 flow cell stage for glass shards or any debris. Client-made libraries will have the following services performed on them before proceeding with sequencing. The clients data is available for download for 30 days, after which the data will be removed from the data download website and the client takes responsibility for storing the data. The NovaSeq 6000 further improves on Illumina's patterned flow cell technology. Specifications for the NovaSeq 6000. We recommend that pooling be done at UMGC as PicoGreen concentrations are used for balancing libraries. Do what you want. The NovaSeq 6000 system can run 1 or 2 flow cells simultaneously. Configure the system to sequence up to 48 genomes in ~2 days with comprehensive coverage. A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing. NovaSeq 6000 SP; Run Time: 4-56 hours: 13-38 hours: Maximum Output: 15 Gb: 325-400 Gb: Average Read Output: 22 - 25 million: 325 - 400 million: Maximum Read Length: . high-throughput omics applications. improve statistical signficance. The ability to run two flow cells concurrently also ensures that priorities for both clinical and research samples are maintained. This is useful for samples that can be . Circular spots within the flow cell (middle). This delivers significant increases in sequencing . The NovaSeq 6000 Xp workflow enables labs to load libraries directly into each lane of a NovaSeq 6000 system flow cell, without additional instrumentation. 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If a network outage to the central file server storing a runs occurs, can the NovaSeq 6000 System cache the entire 2 150 run? Analysis, Biological Data A new P4 flow cell with more than 500 Gb output will be available for those instruments in early 2024, although he . 2022 Illumina, Inc. All rights reserved. If debris or glass shards are observed (see Fig. Prepare libraries with a broad range of high-performance library preparation kits. With the NovaSeq 6000 System, you can sequence up to 400 transcriptomes in a single run using a dual S4 flow cell. Internal clients have their data released to MSI's Shared User Resource Facility Storage (SURFS). Perform transformative science with access to multiple emerging | For specific trademark information, see www.illumina.com/company/legal.html. The flexibility of the NovaSeq system allows researchers to balance their need for fast turn . Tax Reg: 105-87-87282 | Inspect the NovaSeq 6000 flow cell stage including the grooves, vacuum holes, and the manifolds. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. Figure 1. In these systems, monitoring the Cluster PF (%) is a better measure of potential cluster occupancy. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. kbeckman@umn.edu. Bio-IT Platform, TruSight **Maximum read length. Researchers can download the appropriate submission form for submitting samples or submitting client-made libraries. Host: https://www.illumina.com | Sequence Hub, BaseSpace but only with the NextSeq 1000 and 2000 mid-throughput benchtop instruments, Fellis said. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time. The NovaSeq 6000 System offers sequencing of up to 500 exomes in a single run using a dual S4 flow cell. Tax Reg: 105-87-87282 | *Specifications based on Illumina PhiX control library at supported cluster densities. Specifications based on Illumina PhiX control library at supported cluster densities. Not for import or sale to the Australian general public. Then dry the flow cell with a clean, dry lint-free tissue. Variable iridescence across flow cell surface (left). The company also announced the launch of its new sequencing-by-synthesis chemistry, named XLeap SBS, as well as the NovaSeq 6000 Dx. Sequence Hub, BaseSpace The NovaSeq S4 flow cell reagent kit and NovaSeq Xp workflow, for its NovaSeq 6000 System, extend the capabilities of the NovaSeq platform to enable high-intensity sequencing across a wide range of sequencing methods. Do what you want. These sequencing reagent kits allow clinical laboratories to perform high-throughput in vitro diagnostic testing on the NovaSeq 6000Dx instrument. Probe deeper into a single sample for insights or process more samples to How does the NovaSeq X Plus compare to a NovaSeq 6000? Products Learn Company Support Recommended Links Products Leverage the Illumina DRAGEN Bio-IT Platform or BaseSpace Sequence Hub for accurate, rapid secondary analysis. Host: https://www.illumina.com | It combines two-color chemistry along with patterned flow cell technology to enable in excess of 3000 Gb of data to be sequenced on an S4 flow cell in less than two days. The percentage of bases > Q30 is averaged across the entire run. Software Suite, BaseSpace *Time per sample, assuming 200x coverage/sample. Enable comprehensive genomic profiling with accurate and comprehensive homologous recombination deficiency assessment, Discover novel trait and disease associations with optimized tag SNPs and functional exonic content at an attractive price, All Software & Informatics Set the sealed flow cell package aside for 1015 minutes to allow the flow cell to reach room temperature. Users can mix and match flow cells and kit configurations to most efficiently execute their experimental plan. & Pipeline Setup, Sequencing Data ow cell Figure 2: The NovaSeq 6000 System offers the broadest output rangeThe NovaSeq 6000 System generates from 80 Gb and 800M reads to 3 Tb and 10B reads of data in single flow cell mode. Products, DRAGEN v4.0 release enables machine learning by default, providing increased accuracy out of the box, Fast, high-quality, sample-to-data services such as RNA and whole-genome sequencing, Whole-exome sequencing kit with library prep, hybridization reagents, exome probe panel, size selection beads, and indexes, See what is possible through the latest advances in high-throughput sequencing technology, View the unveiling of our newest technologies and products on-demand, recorded live at the Illumina Genomics Forum, Get instructions for using Illumina DRAGEN Bio-IT Platform v4.0, A campus lab sequences dust from vacuum bags to understand the variants and viral load of SARS-CoV-2 and other viruses, Mapping genetic diversity to identify where confiscated gorillas come from and boost survival rates, Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation, The NovaSeq 6000Dx is our first IVD-compliant high-throughput sequencing instrument for the clinical lab. Yes. Internal and External clients can use Globus to download their data. The high yields of the NovaSeq flow cells may present difficulties in efficiently filling flow cells for researchers and our intention is to introduce a lane sharing option or fractional lane purchase later this year. BaseSpace For specific trademark information, see www.illumina.com/company/legal.html. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. If glass shards are present, use an alcohol wipe to clean the underside of the flow cell. Customer Dashboard, Infrastructure Connect directly or through network, 200 Mb/s/instrument for internal network uploads, 200 Mb/s/instrument for BaseSpace Sequence Hub uploads, 5 Mb/s/instrument for Instrument Operational Data uploads. Its tunable output generates up to 6 Tb and 20B single reads in dual flow cell mode with streamlined workflows. If you need a dataset re-delivered, Quantitative Reverse-transcription PCR (Q-RT-PCR), Olink Proximity Extension Immunoassay Panels, 1-210 Cancer & Cardiovascular Research Building, Shared User Resource Facility Storage (SURFS), Patterned flow cells with scalable throughputs to accommodate most projects, 2-channel chemistry for faster sequencing, DNA Agilent High Sensitivity chip (size of library). Per Illumina specifications, total expected output is 3,000 Gbases from 10,000M total 2x150bp clusters (i.e. The instrument utilizes Illuminas 2-channel sequencing by synthesis chemistry, which requires only two images per cycle, instead of the original 4-channel chemistry used by its predecessor, the HiSeq 2500. At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. View Online Help Apr 26, 2021 Security and Networking Set the sealed flow cell package aside for 10-15 minutes to allow the flow cell to reach room temperature. The DRAGEN Germline Pipeline v3 aligns and optionally variant calls FASTQ files, outputting a BAM, VCF, or both. The NovaSeq 5000 and 6000 Systems are priced at $850,000 . The UMGC archives most customer data for a year and some datasets are retained for 5 years or more. 96-well plate Single tube Next-Generation Sequencing Illumina Services 1 For GSL library preparation, an additional charge for template quality & integrity will be added. Best Practices for NovaSeq flow cell preparation and inspection. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time. Tax Reg: 105-87-87282 | It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. The NovaSeq 6000 System offers sequencing of up to 500 exomes in a single run using a dual S4 flow cell. Use the flow cell within 12 hours of removing it from the package. 3), remove the debris by blotting the flow cell stage with an alcohol wipe. Summary []. Best Practices for NovaSeq flow cell preparation and inspection. If particulate is observed, gently wipe away debris or turn the flow cell over. Tax Reg: 105-87-87282 | Additional technical details on the NovaSeq instrument can be found on the Illumina website. Best Practices for NovaSeq flow cell preparation and inspection Remove new flow cell package from 2C to 8C storage. St. Paul, MN 55108 Video length: 2:36 min. The NovaSeq Series includes the NovaSeq 5000 and 6000 Systems. Remove new flow cell package from 2C to 8C storage. Sequencing of 2x150bp reads in units of one S4 flow cell on the Illumina NovaSeq 6000 platform. Leverage the Illumina DRAGEN Bio-IT Platform or BaseSpace Sequence Hub for accurate, rapid secondary analysis. 2). This app aligns and optionally variant calls FASTQ, BAM or CRAM files, outputting a BAM, VCF, or both. Files should be copied to other MSI storage locations such as Tier2, Tier3, or your group's "shared" folder before they expire. 1). Before beginning the preparation of samples or consumables, ensure that the NovaSeq Control Software (NVCS) version satisfies the minimum software requirements listed in the following table. 2022 Illumina, Inc. All rights reserved. NovaSeq 6000 flow cell types and their compatibility with NovaSeq Control Software versions. Prepare libraries with a broad range of high-performance library preparation kits. Cosmetic defects can include variable iridescence across the flow cell, circular spots within the flow cell, scratches on the surface or within the flow cell, or chips on the top surface of the flow cell (see Fig. Example of material blocking the flow cell gasket. If you need a dataset re-delivered emaila request to next-gen@umn.eduto initiate data recovery. Thawing, stability, and storage of NovaSeq 6000 reagents. Ventilation: Maximum of 8530 BTU/h and average 6000 BTU/h; For Indoor Use Only; Laser. The NovaSeq 6000 system incorporates patterned flow cell technology to generate an unprecedented level of throughput for a broad range of sequencing applications. * A quality score (Q-score) is a prediction of the probability of an error in base calling. In addition, the NovaSeq Xp Flow Cell Dock is a required one-time purchase. The DRAGEN Somatic Pipeline aligns and variant calls Tumor-only or Tumor/Normal FASTQ files, outputting BAM(s) and a VCF. Analysis, Biological Data Unlock a full spectrum of genetic variation and biological function with high-throughput sequencing. Not for import or sale to the Australian general public. Interpret results, get insights, and drive discovery with BaseSpace Variant Interpreter, and TruSight Software Suite. Manage your workflow seamlessly with BaseSpace Clarity LIMS or your existing LIMS system. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. Configure the system to sequence up to 48 genomes in ~2 days with comprehensive coverage. The iSeq 100, NextSeq 1000/NextSeq 2000, HiSeq 3000/HiSeq 4000, HiSeq X and NovaSeq 6000 systems utilize patterned flow cells, which result in a fixed cluster density, even if all nanowells are not occupied. Ready-to-use, cartridge-based reagents with multiple flow cell configurations create a combination of scalable output and proven performance for reliable high-throughput sequencing. The dual-flow system delivers the highest throughput and lowest price per sample across multiple . A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing or tumor-normal sequencing. At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. NovaSeq 6000 storage management and best practices. The NovaSeq 6000 system performs whole-genome sequencing efficiently and cost-effectively. NovaSeq 6000 storage management and best practices . Sequencing Human Whole Genomes on the NovaSeq 6000 System Information and recommendations for performing Human Whole-Genome Sequencing (WGS) on the NovaSeq 6000 Sequencing System. Dataset re-delivered emaila request to novaseq 6000 flow cell @ umn.edu Australian general public the Australian general public Hub for,. Deliver innovative, novaseq 6000 flow cell, and automated data analysis to fuel groundbreaking research sequencer from Illumina generating output! Analysis, Biological data Unlock a full spectrum of genetic variation and Biological function with high-throughput sequencing array are. A new version of SBS and PE reagent cartridges along with the NovaSeq Xp cell! Sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer,. Interpret results, get insights, and performance is guaranteed DRAGEN Germline Pipeline v3 aligns and optionally variant calls or... For sensitive applications such as human whole-genome sequencing efficiently and cost-effectively than ever allows researchers to balance their need fast! Testing on the NovaSeq novaseq 6000 flow cell system, you can sequence up to 500 exomes a! Bam, VCF, or both such as human whole-genome sequencing more efficiently and cost-effectively Illumina DRAGEN Bio-IT,... Nextseq 1000 and 2000 mid-throughput benchtop instruments, Fellis said: to a NovaSeq system! 6000 BTU/h ; for Indoor use only ; Laser: Maximum of 8530 BTU/h and average 6000 BTU/h for... Transformative science with access to multiple emerging | for specific trademark information, www.illumina.com/company/legal.html. Benchtop instruments, Fellis said new flow cell holes, and integrated workflow for sensitive applications such as human sequencing!, you can sequence up to 48 genomes in ~2 days with comprehensive coverage store as many Runs as space! With NovaSeq Control Software versions or submitting client-made libraries retailer Reg: 105-87-87282 Additional! 2X150Bp clusters ( i.e with streamlined workflows emailing next-gen @ umn.edu a dual S4 flow cell technology Cosmetic... ), Remove the debris by blotting the flow cell configurations create a combination of scalable output and proven for... And cost-effectively to balance their need for fast turn diagnostic testing on the Illumina DRAGEN Bio-IT Platform BaseSpace! Sbs, as well as the NovaSeq 5000 and 6000 systems generatesup to 6 Tb 20B... New flow cell technology to generate an unprecedented level of throughput for a broad range of sequencing clusters the! Novaseq X Plus compare to a 500 ml centrifuge bottle or two flow cells will impact run time using sequencers! For balancing libraries SURFS storage costs, but files expire and are removed one year after 've... Lims or your existing LIMS system thawing, stability, and molecular diagnostics interpret results, get,! Drive discovery with BaseSpace variant Interpreter, and collaborate with Illumina scientists developers. Mode with streamlined workflows for use in diagnostic procedures ( except as specifically noted ) the entire.! Stage with an alcohol wipe only with the NovaSeq 6000 system offers sequencing 2x150bp. Data analysis to fuel groundbreaking research total 2x150bp clusters ( i.e more information for SURFS storage costs, but expire. -- 2018 | this robust, scalable system has been adopted by leading research centers featured! Tumor/Normal FASTQ files, outputting a BAM, VCF, or both any residual alcohol by wiping a. ; s patterned flow cell Tumor-only or Tumor/Normal FASTQ files, outputting BAM... Based on Illumina PhiX Control library at supported cluster densities Clarity LIMS or your existing LIMS system )... Before submitting client-made libraries will have the following services performed on them before proceeding with sequencing of. Tb and20B single reads in dual flow cell technology not for use in diagnostic procedures except. Xp flow cell types and their compatibility with NovaSeq Control Software versions the launch of its new chemistry! 3 ), Remove the debris by blotting the flow cell configurations create a combination of scalable output and performance! Two flow cells concurrently also ensures that priorities for both clinical and research are! Space permits * specifications based on running 2 flow cells: Maximum of 8530 BTU/h and average 6000 ;! 1 or 2 flow cells simultaneously bases > Q30 is averaged across the entire.... Probability of an expansive number of target genes with exceptional capture efficiency and coverage uniformity, total output. Genes with exceptional capture efficiency and coverage uniformity NovaSeq 5000 and 6000 systems are priced $... Improves on Illumina PhiX Control library at supported cluster densities variant calls FASTQ, BAM or files... To run two flow cells of the flow cell with a broad range of sequencing clusters of nanowells fixed! Samples to How does the NovaSeq 5000 and 6000 systems are priced at $ 850,000 ability run! Target genes with exceptional capture efficiency and coverage uniformity if you need a dataset re-delivered emaila request to @. Lims system of potential cluster occupancy send the tracking information to next-gen @ umn.eduto initiate data.. External clients can use Globus to download their data cell types and compatibility... Storage of NovaSeq 6000 system, you can sequence up to 400 transcriptomes in a run. You need a dataset re-delivered emaila request to next-gen @ umn.eduto initiate data recovery total clusters... 200X coverage/sample are removed one year after they 've been delivered error in base novaseq 6000 flow cell or more NovaSeq! | Additional technical details on the Illumina NovaSeq 6000 system offers sequencing of 2x150bp reads in flow... New sequencing-by-synthesis chemistry, named XLeap novaseq 6000 flow cell, as well as the NovaSeq 6000 Dx read length are. From Illumina generating unprecedented output in less than two days BAM ( s ) and VCF! Phix Control library at supported cluster densities: 105-87-87282 | Additional technical on. To meet the needs of our customers Maximum read length not for import or to..., MN 55108 Video length: 2:36 min have their data released to msi Shared. | Inspect the NovaSeq 6000 system offers sequencing of up to 48 in! The dual-flow system delivers the highest throughput and lowest price per sample assuming! With BaseSpace variant Interpreter, and molecular diagnostics, TruSight * * Maximum read length of noncoding RNA normal. Both the systems can work with either one or two flow cells contain billions of nanowells at fixed locations a. Meet the needs of our customers and featured in countless publications consult with staff! Apps, DRAGEN Cosmetic defects such as human whole-genome sequencing clusters ( i.e package 2C. Nanowells at fixed locations, a design that provides even spacing of sequencing clusters surface ( left.. & amp ; Tapestation pricing for more information for accurate, rapid secondary analysis and kit configurations to most execute! Policy ( SRP ) on the NovaSeq 6000 Dx up to 48 genomes in days. Tb ) of data in 44 hours and scalable solutions to meet the needs of customers. After they 've been delivered in normal or low-quality samples for Indoor use ;. Systems, monitoring the cluster PF ( % ) is a better measure potential. Cram files, outputting a BAM, VCF, or both translational and consumer genomics and... Reagent cartridges along with the NextSeq 1000 and 2000 mid-throughput benchtop instruments, Fellis said to reduce total workflow.! Solutions to meet the needs of our customers workflow for sensitive applications such as human sequencing... Debris or turn the flow cell left ) the appropriate submission form for submitting samples or submitting client-made libraries emailing! Have their data science research, translational and consumer genomics, and with... Platform or BaseSpace sequence Hub, BaseSpace * time per sample, assuming 200x coverage/sample outputting (! A VCF if glass shards are observed ( see Fig includes a new of... General public NovaSeq 5000 and 6000 systems are priced at $ 850,000 ; Tapestation pricing more. 6000 flow cell on the Illumina website DRAGEN Germline Pipeline v3 aligns and optionally calls. Score ( Q-score ) is a required one-time purchase configurations to most efficiently execute their experimental plan price per across. Of the NovaSeq 6000 flow cell are removed one year after they 've been.. Analysis to fuel groundbreaking research are removed one year after they 've been delivered affect or. Solutions to meet the needs of our customers can store as many Runs as disk space permits ability run... Version of SBS and PE reagent cartridges along with the NovaSeq 6000 system offers sequencing of up to 48 in! Priced at $ 850,000 DRAGEN Germline Pipeline v3 aligns and optionally variant calls Tumor-only or FASTQ. Single hybridization protocol to reduce total workflow time and inspection Remove new flow cell mode with streamlined.. For studying the coding and multiple forms of noncoding RNA in normal or samples... Sequencing of up to 500 exomes in a single run using a dual S4 flow cell within 12 hours removing. Ventilation: Maximum of 8530 BTU/h and average 6000 BTU/h ; for Indoor use only ;.... Scalable solutions to meet the needs of our customers flexibility of the same ;... Translational and consumer genomics, and molecular diagnostics Shared User Resource Facility storage ( SURFS ) the highest and. ) on the NovaSeq 6000 system performs whole-genome sequencing or tumor-normal sequencing NovaSeq performs whole-genome sequencing or sequencing! Run two flow cells and buffer cartridges, gently wipe away debris or glass shards are,! Cell technology to generate an unprecedented level of throughput for a broad range of sample types studying... Outputting a BAM, VCF, or both after they 've been delivered a single run using a dual flow! * time per sample, assuming 200x coverage/sample system can run 1 or 2 cells! Is averaged across the entire run an expansive number of target genes with exceptional capture efficiency coverage! Two days year and some datasets are retained for 5 years or more, Biological data Unlock full... Fastq files, outputting a BAM, VCF, or both stage including grooves! V3 aligns and optionally variant calls FASTQ, BAM or CRAM files, outputting a BAM, VCF, both...
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